肠道腺苷三磷酸酶的研究。I. 一种半自动方法在大鼠肠道刷状缘的应用。
Studies on intestinal adenosine triphosphatases. I. Application of a semiautomated method to the rat intestinal brush borders.
作者信息
Ferard G, Sall I, Metais P
出版信息
Enzyme. 1975;19(1):38-47.
PMID:124250
Abstract
Elaboration of a semiautomated kinetic test on LKB 8600 apparatus for ATPase is described, using the PK-LDH system. As optimal ionic conditions 3 mmol-1 - minus 1 potassium chloride and 100 mmol-1 - minus 1 sodium chloride are proposed for measurement of (Na+-K+)-ATPase activities of rat intestinal brush borders. NH+4 can substitute for K+. The coefficients of variation of the method are 2.4% for Mg2+-ATPase and 4.9% for (Na+-K+)-ATPase determinations.
摘要
本文描述了一种利用PK-LDH系统在LKB 8600仪器上进行ATP酶半自动动力学测试的方法。对于大鼠肠刷状缘（Na⁺-K⁺）-ATP酶活性的测定，建议使用3 mmol⁻¹ 氯化钾和100 mmol⁻¹ 氯化钠作为最佳离子条件。NH₄⁺ 可以替代K⁺。该方法的变异系数在测定Mg²⁺-ATP酶时为2.4%，在测定（Na⁺-K⁺）-ATP酶时为4.9%。
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