Crescenzi Vittorio, Francescangeli Andrea, Taglienti Anna
Department of Chemistry, University La Sapienza, Rome, Italy.
Biomacromolecules. 2002 Nov-Dec;3(6):1384-91. doi: 10.1021/bm025657m.
New types of hydrogels have been obtained starting from high bloom purified gelatin A, alone or in mixtures with hyaluronan and with a hyaluronan derivative bearing primary amino groups, by transglutaminase-catalyzed cross-linking. The reticulation process, carried out adopting two different temperature protocols, and the ensuing materials have been characterized in terms of rheologically estimated gel times, equilibrium swelling in water and in phosphate buffer solution (PBS), and rigidity modulus. Main structural and conformational factors governing the physicochemical properties and the possible application of the new hydrogels are discussed.
通过转谷氨酰胺酶催化交联，从高冻力纯化明胶A单独或与透明质酸以及带有伯氨基的透明质酸衍生物的混合物开始，已获得新型水凝胶。采用两种不同温度方案进行的交联过程以及所得材料已根据流变学估计的凝胶时间、在水和磷酸盐缓冲溶液（PBS）中的平衡溶胀以及刚性模量进行了表征。讨论了控制新型水凝胶物理化学性质和可能应用的主要结构和构象因素。
