Feng Yunzhi, Ling Tianyou, Wu Hanjiang, Li Yunliang, Liu Zhiwen
Department of Prosthodonties, Peking University School of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2002 Sep;37(5):361-2.
To investigate the effects of cyclosporin A (CSA) on the level of intercellular adhesion molecule-1 (ICAM-1) expressed by oral fibroblasts (FB).
The fibroblasts were obtained from normal buccal mucosa (NM-FB) and cultured in vitro. Then the levels of ICAM-1 expressed by fibroblasts incubated with or without CSA in the presence of 10% fetal calf serum for 48 hours at 37 degrees C in 5% CO(2) and air were monitored by using cell-based ELISA for ICAM-1.
We demonstrated that NM-FB had ICAM-1 expression (A = 0.324 +/- 0.030), and after 48 hours incubated with CSA the ICAM-1 levels expressed by oral buccal mucosa fibroblasts were dose-dependently decreased.
CSA reduces the levels of ICAM-1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.
研究环孢素A（CSA）对口腔成纤维细胞（FB）表达细胞间黏附分子-1（ICAM-1）水平的影响。
从正常颊黏膜获取成纤维细胞（NM-FB）并进行体外培养。然后将在含有10%胎牛血清的条件下，于37℃、5%二氧化碳和空气环境中分别与CSA共同孵育或不与CSA共同孵育48小时的成纤维细胞所表达的ICAM-1水平，通过基于细胞的ICAM-1酶联免疫吸附测定法进行监测。
我们证明NM-FB有ICAM-1表达（A = 0.324 ± 0.030），并且在与CSA孵育48小时后，口腔颊黏膜成纤维细胞所表达的ICAM-1水平呈剂量依赖性降低。
CSA降低口腔黏膜FB表达的ICAM-1水平，可能对某些口腔黏膜疾病的治疗有用。
