胰岛素反应性葡萄糖转运蛋白——GLUT8和GLUT4在发育中的哺乳动物大脑中表达。

Insulin-responsive glucose transporters-GLUT8 and GLUT4 are expressed in the developing mammalian brain.

作者信息

Sankar Raman, Thamotharan Shanthie, Shin Don, Moley Kelle H, Devaskar Sherin U

机构信息

Department of Pediatrics, UCLA School of Medicine, Los Angeles, CA 90095-1752, USA.

出版信息

Brain Res Mol Brain Res. 2002 Nov 15;107(2):157-65. doi: 10.1016/s0169-328x(02)00487-4.

DOI:10.1016/s0169-328x(02)00487-4

PMID:12425944

Abstract

We investigated the spatial and temporal distribution of insulin-responsive facilitative glucose transporter isoforms GLUT4 and GLUT8 in the developing mouse brain. Employing Western blot analysis and specific antibodies, GLUT4 and GLUT8 peaked during the suckling phase. Immunohistochemical analysis revealed the presence of GLUT4 mainly in neurites in sensory and motor areas of cortical and subcortical structures of the brain from P7 until adulthood. In contrast, GLUT8 was found in the same anatomical structures within neurites and cell bodies. Most striking was the presence of GLUT8 in the cell bodies of the substantia nigra. We conclude that both GLUT4 and GLUT8 are present in murine brain, with highest concentrations noted during the suckling phase. These insulin-responsive isoforms may have a unique role in augmenting substrate delivery under conditions of increased demand.

摘要

我们研究了胰岛素反应性易化葡萄糖转运体亚型GLUT4和GLUT8在发育中小鼠大脑中的时空分布。利用蛋白质印迹分析和特异性抗体，发现GLUT4和GLUT8在哺乳期达到峰值。免疫组织化学分析显示，从出生后第7天（P7）到成年期，GLUT4主要存在于大脑皮质和皮质下结构的感觉和运动区域的神经突中。相比之下，GLUT8在相同解剖结构的神经突和细胞体中均有发现。最引人注目的是，在黑质的细胞体中存在GLUT8。我们得出结论，GLUT4和GLUT8均存在于小鼠大脑中，在哺乳期浓度最高。这些胰岛素反应性亚型在需求增加的情况下增强底物输送方面可能具有独特作用。