Villanueva V R, Lederer E
FEBS Lett. 1975 Apr 1;52(2):308-11. doi: 10.1016/0014-5793(75)80832-5.
A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazone back to epsilon-N-trimethyllysine by hydrogenolysis in a Parr bomb. Analyses on TLC and in four different ion exchange chromatographic systems show the appearance of a ninhydrin positive product having the same Rf and the same retention time as epsilon-N-trimethyllysine; it contains more than 85% of the radioactivity of the 2,4-dinitrophenylhydrazone of the keto acid.
一种新的ε-N-三甲基赖氨酸代谢产物已从粗糙脉孢菌的菌丝体中分离出来。通过在帕尔弹中进行氢解将其2,4-二硝基苯腙还原回ε-N-三甲基赖氨酸，已确定体内和体外由ε-N-三甲基（[14-C]H3）L-赖氨酸孵育产生的标记化合物为2-酮-ε-N-三甲基己酸。在薄层色谱法（TLC）和四种不同的离子交换色谱系统上的分析表明，出现了一种茚三酮阳性产物，其比移值（Rf）和保留时间与ε-N-三甲基赖氨酸相同；它含有酮酸2,4-二硝基苯腙放射性的85%以上。
