Ning Yun-Shan, Zhou Hong-Wei, Zhou Ming-Qian, Chen Ze-Hong, Fang Xiang-Dong, Fu Ning, Wang Xiao-Ning
Institute of Molecular Immunology, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2001;21(12):881-884.
To study the role of thrombopoietin (TPO) gene 5' untranslated region (UTR) and the intron in TPO expression regulation. METHODS: With the mRNA derived from Chinese human fetal liver as the template, full-length TPO cDNA (approximately 1.1 kb) and TPO genomic DNA(6.2 kb) along with all the introns of TPO gene were isolated by PCR and long-distance PCR techniques from Chinese fetal liver tissue. RESULT: Sequencing analysis indicated that all the coding sequences and the exon/intron splice sites were consistent with the results previously reported by Sohma et al except for only a DNA and all the introns in the intron of TPO gene. CONCLUSION: We have successfully cloned full-length TPO cDNA, TPO genomic DNA and all the introns of TPO gene from Chinese fetal liver tissue.
研究血小板生成素（TPO）基因5′非翻译区（UTR）及内含子在TPO表达调控中的作用。方法：以中国人胎儿肝脏来源的mRNA为模板，采用PCR和长距离PCR技术从中国人胎儿肝脏组织中分离出全长TPO cDNA（约1.1 kb）、TPO基因组DNA（6.2 kb）以及TPO基因的所有内含子。结果：序列分析表明，除TPO基因内含子中的一段DNA及所有内含子外，所有编码序列和外显子/内含子剪接位点均与Sohma等人先前报道的结果一致。结论：我们已成功从中国人胎儿肝脏组织中克隆出全长TPO cDNA、TPO基因组DNA及TPO基因的所有内含子。
