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从嗜热栖热放线菌NRS 2004/3a中分离糖心磷脂。

Isolation of glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a.

作者信息

Schäffer Christina, Beckedorf Anke I, Scheberl Andrea, Zayni Sonja, Peter-Katalinić Jasna, Messner Paul

机构信息

Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, A-1180 Vienna, Austria.

出版信息

J Bacteriol. 2002 Dec;184(23):6709-13. doi: 10.1128/JB.184.23.6709-6713.2002.

DOI:10.1128/JB.184.23.6709-6713.2002
PMID:12426359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC135447/
Abstract

Glucose-substituted cardiolipins account for about 4 mol% of total phospholipid extracted from exponentially grown cells of Geobacillus stearothermophilus NRS 2004/3a. Individual glucocardiolipin species exhibited differences in fatty acid substitution, with iso-C(15:0) and anteiso-C(17:0) prevailing. The compounds were purified to homogeneity by a novel protocol and precharacterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

摘要

葡萄糖取代的心磷脂约占从嗜热栖热放线菌NRS 2004/3a指数生长期细胞中提取的总磷脂的4摩尔%。各个葡萄糖心磷脂种类在脂肪酸取代方面存在差异，其中异-C(15:0)和anteiso-C(17:0)占主导。通过一种新方法将这些化合物纯化至同质，并通过基质辅助激光解吸电离飞行时间质谱进行预表征。

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本文引用的文献

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2
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J Biol Chem. 2002 Feb 22;277(8):6230-9. doi: 10.1074/jbc.M108873200. Epub 2001 Dec 10.
3
Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th.嗜热需氧芽孢杆菌的分类学研究：地下嗜热栖热菌新属、新种及乌津嗜热栖热菌新种的描述，从石油储层分离，以及嗜热脂肪芽孢杆菌、热链芽孢杆菌、嗜热油芽孢杆菌、嗜碱芽孢杆菌、热脱硝芽孢杆菌转移至嗜热栖热菌属成为新组合嗜热脂肪栖热菌、嗜热链栖热菌等。
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S-layer-supported lipid membranes.S层支撑的脂质膜。
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