从嗜热栖热放线菌NRS 2004/3a中分离糖心磷脂。
Isolation of glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a.
作者信息
Schäffer Christina, Beckedorf Anke I, Scheberl Andrea, Zayni Sonja, Peter-Katalinić Jasna, Messner Paul
机构信息
Zentrum für Ultrastrukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur Wien, A-1180 Vienna, Austria.
出版信息
J Bacteriol. 2002 Dec;184(23):6709-13. doi: 10.1128/JB.184.23.6709-6713.2002.
Abstract
Glucose-substituted cardiolipins account for about 4 mol% of total phospholipid extracted from exponentially grown cells of Geobacillus stearothermophilus NRS 2004/3a. Individual glucocardiolipin species exhibited differences in fatty acid substitution, with iso-C(15:0) and anteiso-C(17:0) prevailing. The compounds were purified to homogeneity by a novel protocol and precharacterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
摘要
葡萄糖取代的心磷脂约占从嗜热栖热放线菌NRS 2004/3a指数生长期细胞中提取的总磷脂的4摩尔%。各个葡萄糖心磷脂种类在脂肪酸取代方面存在差异，其中异-C(15:0)和anteiso-C(17:0)占主导。通过一种新方法将这些化合物纯化至同质，并通过基质辅助激光解吸电离飞行时间质谱进行预表征。
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