Redzko Slawomir, Przepiesc Jerzy, Zak Janusz, Turowski Dariusz, Urban Jan, Wysocka Jolanta
Klinika Perinatologii, Akademia Medyczna, ul. Curie-Sklodowskiej 24a, Bialystok, Poland.
Med Wieku Rozwoj. 2002 Apr-Jun;6(2):145-53.
Reticulocyte analysis using flow cytometry increases the precision of reticulocyte enumeration and can evaluate the maturation of reticulocytes. Reticulocytes count and their subtypes may reflect function of hematopoesis, especially during hypoxia. This study was aimed to establish reticulocyte count and reticulocyte maturation profile in cord blood during normal pregnancy and pregnancy complicated by chronic intrauterine hypoxia. For this purpose we analysed 233 cord blood specimens derived from uneventful pregnancy and 58 cord blood specimens from complicated pregnancy. The gestational age ranged from 26 to 42 weeks. The following measurements were obtained (mean +/- standard deviation): reticulocyte absolute count - 268 +/- 63 10(9)/l, reticulocyte percentage - 6.4 +/- 1.5; high fluorescence reticulocyte HFR - 11.3 +/- 3.8%; medium reticulocyte fraction MFR - 18.1 +/- 4.7%; low reticulocyte fraction LFR - 70.7 +/- 7%; immature reticulocyte fraction IFR - 29.3 +/- 7%. The percentage values of reticulocytes decreased in cord blood according to gestational age increase, but maturation subpopulations did not change significantly. Reticulocyte fractions in normal pregnancy were different compared to complicated pregnancy.
使用流式细胞术进行网织红细胞分析可提高网织红细胞计数的精度，并能评估网织红细胞的成熟情况。网织红细胞计数及其亚型可能反映造血功能，尤其是在缺氧期间。本研究旨在确定正常妊娠和合并慢性宫内缺氧妊娠时脐带血中网织红细胞计数及网织红细胞成熟谱。为此，我们分析了233份来自正常妊娠的脐带血标本和58份来自合并妊娠的脐带血标本。孕周范围为26至42周。获得以下测量结果（平均值±标准差）：网织红细胞绝对计数 - 268±63×10⁹/L，网织红细胞百分比 - 6.4±1.5%；高荧光网织红细胞HFR - 11.3±3.8%；中网织红细胞比例MFR - 18.1±4.7%；低网织红细胞比例LFR - 70.7±7%；未成熟网织红细胞比例IFR - 29.3±7%。脐带血中网织红细胞的百分比值随孕周增加而降低，但成熟亚群无明显变化。正常妊娠与合并妊娠的网织红细胞比例不同。
