Asada Yoko, Kawamoto Yoshi, Shotake Takayoshi, Terao Keiji
Tsukuba Primate Center, National Institute of Infectious Diseases, 1-Hachimandai, Tsukuba, Ibaraki 305-0843, Japan.
Primates. 2002 Oct;43(4):343-9. doi: 10.1007/BF02629608.
The cross-reactivity of five different rabbit polyclonal antibodies to human IgG and IgG subclass (IgG1, IgG2, IgG3, and IgG4) was determined by competitive ELISA with nine nonhuman primate species including five apes, three Old World monkeys, and one New World monkey. As similar to those previously reported, the reactivity of anti-human IgG antibody with plasma from different primate species was closely related with phylogenic distance from human. Every anti-human IgG subclass antibody showed low cross-reactivity with plasma from Old World and New World monkeys. The plasma from all apes except for gibbons (Hylobates spp.) showed 60 to 100% of cross-reactivity with anti-human IgG2 and IgG3 antibodies. On the other hand, chimpanzee (Pan troglodytes and Pan paniscus) and orangutan (Pongo pygmaeus) plasma showed 100% cross-reactivity with anti-human IgG1 antibody, but gorilla (Gorilla gorilla) and gibbon plasma showed no cross-reactivity. The chimpanzee and gorilla plasma cross-reacted with anti-human IgG4 antibody at different reactivity, 100% in chimpanzee and 50% in gorilla, but no cross-reactivity was observed in orangutan and gibbon plasma. These results suggest the possibilities that the divergence of "human-type" IgG subclasses might occur at the time of divergence of Homo sapience from Hylobatidae, and that the molecular evolution of IgG1 as well as IgG4 is different from that of IgG2 and IgG3 in great apes, this is probably caused by different in development of immune function in apes during the course of evolution.
通过竞争性酶联免疫吸附测定（ELISA），利用包括5种猿类、3种旧世界猴和1种新世界猴在内的9种非人类灵长类动物，测定了5种不同兔抗人IgG及IgG亚类（IgG1、IgG2、IgG3和IgG4）多克隆抗体的交叉反应性。与先前报道的结果相似，抗人IgG抗体与不同灵长类动物血浆的反应性与它们和人类的系统发育距离密切相关。每种抗人IgG亚类抗体与旧世界猴和新世界猴的血浆均显示出低交叉反应性。除长臂猿（长臂猿属）外，所有猿类的血浆与抗人IgG2和IgG3抗体均显示出60%至100%的交叉反应性。另一方面，黑猩猩（黑猩猩和倭黑猩猩）和猩猩（婆罗洲猩猩）的血浆与抗人IgG1抗体显示出100%的交叉反应性，但大猩猩（大猩猩）和长臂猿的血浆则无交叉反应性。黑猩猩和大猩猩的血浆与抗人IgG4抗体发生不同反应性的交叉反应，黑猩猩为100%，大猩猩为50%，但猩猩和长臂猿的血浆未观察到交叉反应性。这些结果提示，“人类型”IgG亚类的分化可能发生在智人与长臂猿科分化之时，并且在大猩猩中，IgG1以及IgG4的分子进化不同于IgG2和IgG3，这可能是由猿类在进化过程中免疫功能发育的差异所导致的。
