Spada R G M, da Silva D, Abdel-Hamid A-Z, Sobral-Hamaguchi S S, Zuim N R B, Zanotti-Magalhaes E M, Magalhaes L A, Ribeiro-Paes J T
Departamento de Ciências Biológicas, Unesp, Assis, SP, 19806-900, Brasil.
Mem Inst Oswaldo Cruz. 2002;97 Suppl 1:53-8. doi: 10.1590/s0074-02762002000900012.
The analysis of the genetic variability related to susceptibility to Schistosoma mansoni infection in the vector of the genus Biomphalaria is important in terms of a better understanding of the epidemiology of schistosomiasis itself, the possible pathological implications of this interaction in vertebrate hosts, and the formulation of new strategies and approaches for disease control. In the present study, the genetic variability of B. glabrata strains found to be resistant or susceptible to S. mansoni infection was investigated using DNA amplification by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The amplification products were analyzed on 8% polyacrylamide gel and stained with silver. We selected 10 primers, since they have previously been useful to detect polymorphism among B. glabrata and/or B. tenagophila. The results showed polymorphisms with 5 primers. Polymorphic bands observed only in the susceptible strain. The RAPD-PCR methodology represents an adequate approach for the analysis of genetic polymorphisms. The understanding of the genetic polymorphisms associated to resistance may contribute to the future identification of genomic sequences related to the resistance/susceptibility of Biomphalaria to the larval forms of S. mansoni and to the development of new strategies for the control of schistosomiasis.
对双脐螺属病媒中与曼氏血吸虫感染易感性相关的遗传变异性进行分析，对于更好地理解血吸虫病本身的流行病学、这种相互作用在脊椎动物宿主中可能产生的病理影响以及制定新的疾病控制策略和方法具有重要意义。在本研究中，使用随机扩增多态性DNA - 聚合酶链反应（RAPD - PCR）进行DNA扩增，对发现对曼氏血吸虫感染具有抗性或易感性的光滑双脐螺菌株的遗传变异性进行了研究。扩增产物在8%聚丙烯酰胺凝胶上进行分析并用银染色。我们选择了10种引物，因为它们此前已被用于检测光滑双脐螺和/或嗜气管双脐螺之间的多态性。结果显示5种引物存在多态性。仅在易感菌株中观察到多态性条带。RAPD - PCR方法是分析遗传多态性的一种合适方法。对与抗性相关的遗传多态性的理解可能有助于未来鉴定与双脐螺对曼氏血吸虫幼虫形式的抗性/易感性相关的基因组序列，并有助于制定控制血吸虫病的新策略。
