Cervenková K, Belejova M, Veselý J, Chmela Z, Rypka M, Ulrichová J, Modrianský M, Maurel P
Department of Pathological Physiology, Medical Faculty, Palacký University, Hnĕvotínská 3, 775 15 Olomouc, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2001 Dec;145(2):57-60. doi: 10.5507/bp.2001.012.
In vitro subcellular and cellular systems have important and irreplaceable roles in the metabolic investigations that precede the development of new potential drugs. Of these model systems, tissue slices are probably the nearest to in vivo conditions. From the experimental and complexity points of view, perfused organs lie midway between tissue slices and whole organism. Preparation and working with liver slices is quick and easy, and, excess material can be cryopreserved and stored untill the next experiment. Slices can be prepared from a wide variety of organs and it is possible to co-incubate them. Another important feature is the possibility of interspecies comparison of slices. Different experiments can be run both in the short-term as well as long-term incubations. Each in vitro system has an important place for example, in the development of new medicaments. It is therefore important to compare and supplement experimental results from different in vitro systems when extrapolating to in vivo situations is done.
体外亚细胞和细胞系统在新潜在药物开发之前的代谢研究中具有重要且不可替代的作用。在这些模型系统中，组织切片可能最接近体内条件。从实验和复杂性的角度来看，灌注器官处于组织切片和整个生物体之间。制备肝切片并进行相关操作快速简便，多余的材料可以冷冻保存，直到下一次实验。切片可以从多种器官制备，并且可以将它们共同孵育。另一个重要特征是可以对不同物种的切片进行比较。短期和长期孵育都可以进行不同的实验。例如，每个体外系统在新药物的开发中都有重要地位。因此，在进行体内情况的推断时，比较和补充来自不同体外系统的实验结果非常重要。
