Psotová J, Zahálková J, Hrbác J, Simánek V, Bartek J
Institute of Medical Chemistry and Biochemistry, Palacký University, 775 15 Olomouc, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2001 Dec;145(2):81-3.
The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from two types of patients. The first one consisted of 29 volunteers (men aged 18-21 years) who were administered placebo or silymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statistically significant increase in total antioxidant capacity compared to placebo. No statistically significant changes in TBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under study comprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease of total antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV was performed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, and a reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple and relatively reliable method for assessment of body antioxidant status. It is also time and cost effective.
循环伏安法（CV）用于测量两类患者的血浆总抗氧化能力。第一类包括29名志愿者（年龄在18 - 21岁的男性），他们被给予安慰剂或剂量为858毫克/天的水飞蓟宾。在给予水飞蓟宾两个月后，循环伏安法显示与安慰剂相比，总抗氧化能力有统计学上的显著增加。未发现硫代巴比妥酸反应物（TBARS）、巯基、肌酐、尿素和尿酸浓度有统计学上的显著变化。研究的第二组包括49名接受透析治疗的慢性肾病患者。透析后，循环伏安法显示血浆中总抗氧化能力下降，这与肌酐、尿素和尿酸的下降相当。循环伏安法使用由工作玻碳电极、辅助铂电极和参比饱和甘汞电极组成的系统进行；施加200 mV/s的线性电压变化。循环伏安法是评估身体抗氧化状态的一种简单且相对可靠的方法。它也是具有时间和成本效益的。
