Ochiai K, Handharyani E, Umemura T
Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Avian Pathol. 2002 Oct;31(5):425-8. doi: 10.1080/0307945021000005789.
Immunohistochemical examinations of hepatic stellate cells (HSCs) were performed on six enlarged livers from broiler chickens with malformation of the extrahepatic biliary tract (group 1) and on eight broiler livers affected with naturally occurring cholangiohepatitis without biliary malformation (group 2). The livers from both groups were grossly enlarged, firm and tan-coloured, and histologically revealed severe diffuse fibrosis with proliferation of bile ductules. HSCs positive for muscle actin and desmin actively proliferated in the perisinusoidal space and around newly formed bile ductules. There was no difference in the immunohistochemical reactivities and location of HSCs between the two groups. The findings suggest that the diffuse hepatic fibrosis found in group 2 as well as group 1 results from reactive proliferation of HSCs.
对6只患有肝外胆道畸形的肉鸡肿大肝脏（第1组）和8只患有自然发生的胆管肝炎且无胆道畸形的肉鸡肝脏（第2组）进行了肝星状细胞（HSCs）的免疫组织化学检查。两组肝脏均明显肿大、质地坚硬且呈棕褐色，组织学检查显示有严重的弥漫性纤维化伴胆小管增生。肌动蛋白和结蛋白阳性的肝星状细胞在窦周间隙和新形成的胆小管周围活跃增殖。两组之间肝星状细胞的免疫组织化学反应性和位置没有差异。这些发现表明，第2组以及第1组中发现的弥漫性肝纤维化是由肝星状细胞的反应性增殖引起的。
