恶性疟原虫：对巴西亚马逊地区自然分离株中转录的var基因序列的分析。

Plasmodium falciparum: analysis of transcribed var gene sequences in natural isolates from the Brazilian Amazon region.

作者信息

Afonso Nogueira Paulo, Wunderlich Gerhard, Shugiro Tada Mauro, d'Arc Neves Costa Joana, José Menezes Maria, Scherf Artur, Pereira-da-Silva Luiz Hildebrando

机构信息

Centro de Pesquisa em Medicina Tropical, CP 87, Correio Central, 78.910-210 Porto Velho, Rondônia, Brazil.

出版信息

Exp Parasitol. 2002 Jun-Jul;101(2-3):111-20. doi: 10.1016/s0014-4894(02)00107-8.

DOI:10.1016/s0014-4894(02)00107-8

PMID:12427465

Abstract

Parasite isolates from Brazilian Western Amazonian patients suffering from uncomplicated falciparum malaria were matured in vitro and their var gene transcripts were analysed by RT-PCR and sequencing. Additionally, the cytoadherence patterns of these isolates were determined by panning techniques using transfected CHO cell lines expressing different surface receptors. All of the isolates tested showed between 4 and 13 different var gene transcripts per isolate. Several of these transcripts were present in more than one isolate and three sequences appeared to be preferentially expressed in natural infections. In most of the isolates, cytoadherence occurred to the receptors ICAM-1 and CD36. Several isolates showed a multiadherent profile. Analysis of MSP1 and MSP2 allelic polymorphism indicated polyclonal infections, that could be responsible for the multiadherent phenotype.

摘要

从巴西亚马逊地区西部患单纯性恶性疟的患者身上分离出的疟原虫在体外培养成熟，通过逆转录聚合酶链反应（RT-PCR）和测序分析其var基因转录本。此外，使用表达不同表面受体的转染中国仓鼠卵巢（CHO）细胞系，通过淘选技术确定这些分离株的细胞黏附模式。所有测试的分离株每个分离株显示出4至13种不同的var基因转录本。其中几种转录本存在于不止一个分离株中，并且三个序列似乎在自然感染中优先表达。在大多数分离株中，细胞黏附发生于细胞间黏附分子-1（ICAM-1）和血小板糖蛋白IV（CD36）受体。几个分离株显示出多黏附特征。对裂殖子表面蛋白1（MSP1）和裂殖子表面蛋白2（MSP2）等位基因多态性的分析表明存在多克隆感染，这可能是多黏附表型的原因。