Yamamoto Ichiro, Tsukada Akira, Saito Noboru, Shimada Kiyoshi
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
Biochim Biophys Acta. 2002 Dec 12;1579(2-3):185-8. doi: 10.1016/s0167-4781(02)00511-0.
We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.
我们报道了鸡转化因子2（Tra 2）cDNA的克隆，该cDNA编码一种由289个氨基酸组成的蛋白质，与哺乳动物剪接因子Tra 2的氨基酸序列具有97.9%的同一性。通过Northern印迹分析，在鸡胚胎组织中检测到Tra 2 mRNA的表达，呈现出一条1.5 kb的条带。整体原位杂交显示，早在孵化第3.5天时，Tra 2 mRNA就在端脑、下颌骨、舌骨弓、翅芽和腿芽中表达。这些结果表明，Tra 2基因可能在鸡胚胎的器官发生中发挥作用。
