鸡胚中Transformer 2（Tra 2）的cDNA克隆及mRNA表达

cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo.

作者信息

Yamamoto Ichiro, Tsukada Akira, Saito Noboru, Shimada Kiyoshi

机构信息

Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.

出版信息

Biochim Biophys Acta. 2002 Dec 12;1579(2-3):185-8. doi: 10.1016/s0167-4781(02)00511-0.

DOI:10.1016/s0167-4781(02)00511-0

PMID:12427554

Abstract

We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.

摘要

我们报道了鸡转化因子2（Tra 2）cDNA的克隆，该cDNA编码一种由289个氨基酸组成的蛋白质，与哺乳动物剪接因子Tra 2的氨基酸序列具有97.9%的同一性。通过Northern印迹分析，在鸡胚胎组织中检测到Tra 2 mRNA的表达，呈现出一条1.5 kb的条带。整体原位杂交显示，早在孵化第3.5天时，Tra 2 mRNA就在端脑、下颌骨、舌骨弓、翅芽和腿芽中表达。这些结果表明，Tra 2基因可能在鸡胚胎的器官发生中发挥作用。