Reichlin Morris, Fesmire James, Quintero-Del-Rio Ana I, Wolfson-Reichlin Marianne
Oklahoma Medical Research Foundation and Oklahoma University College of Medicine, Oklahoma City, OK 73104, USA.
Arthritis Rheum. 2002 Nov;46(11):2957-63. doi: 10.1002/art.10624.
To demonstrate the binding of bovine lipoprotein lipase (LPL) by IgG from sera obtained from patients with systemic lupus erythematosus (SLE) and other rheumatic diseases, and the relationship of anti-LPL to triglyceride levels in SLE.
Binding of LPL by IgG from sera obtained from patients with SLE and other rheumatic diseases was measured by an enzyme-linked immunosorbent assay technique. Lipid profiles for fasting blood samples obtained from SLE patients and control subjects were determined.
Sera obtained from 105 patients with SLE were assessed for reactivity with LPL, and 49 (47%) of the results were positive. Sera obtained from patients with rheumatoid arthritis (RA) (n = 80), Sjögren's syndrome (n = 30), polymyositis and dermatomyositis (n = 30), and progressive systemic sclerosis (n = 31) were also studied, and 10 (13%), 3 (10%), 12 (40%), and 13 (42%), respectively, were positive for reactivity with LPL. It was determined that all affinity-purified anti-double-stranded DNA (dsDNA) antibodies and 4 of 5 monoclonal anti-dsDNA antibodies bound to LPL. The binding of IgG depleted of anti-dsDNA to LPL indicates a second anti-LPL activity in SLE. Measurements of fasting lipid levels in SLE patients with anti-LPL revealed a strong positive correlation of antibody levels and total serum triglycerides, apolipoprotein B, and apolipoprotein E concentrations.
Antibodies to LPL occurred in 47% of SLE patients and in a similar percentage of patients with polymyositis or systemic sclerosis. The prevalence of these antibodies was less in patients with RA or Sjögren's syndrome. It is hypothesized that the elevated triglyceride levels in SLE patients are in part attributable to anti-LPL, and this lipid abnormality could contribute to the premature atherosclerosis known to be present in patients with SLE.
证明系统性红斑狼疮（SLE）及其他风湿性疾病患者血清中的IgG与牛脂蛋白脂肪酶（LPL）的结合情况，以及SLE中抗LPL与甘油三酯水平的关系。
采用酶联免疫吸附测定技术检测SLE及其他风湿性疾病患者血清中IgG与LPL的结合情况。测定SLE患者和对照受试者空腹血样的血脂谱。
对105例SLE患者的血清进行LPL反应性评估，49例（47%）结果为阳性。还研究了类风湿关节炎（RA）患者（n = 80）、干燥综合征患者（n = 30）、多发性肌炎和皮肌炎患者（n = 30）以及进行性系统性硬化症患者（n = 31）的血清，与LPL反应性呈阳性的分别为10例（13%）、3例（10%）、12例（40%）和13例（42%）。已确定所有亲和纯化的抗双链DNA（dsDNA）抗体以及5种单克隆抗dsDNA抗体中的4种与LPL结合。去除抗dsDNA的IgG与LPL的结合表明SLE中存在第二种抗LPL活性。对有抗LPL的SLE患者空腹血脂水平的测量显示，抗体水平与血清总甘油三酯、载脂蛋白B和载脂蛋白E浓度呈强正相关。
47%的SLE患者以及类似比例的多发性肌炎或系统性硬化症患者体内存在抗LPL抗体。这些抗体在RA或干燥综合征患者中的患病率较低。据推测，SLE患者甘油三酯水平升高部分归因于抗LPL，这种脂质异常可能导致SLE患者中已知存在的过早动脉粥样硬化。
