Alcolea Jesús F, Cano Antonio, Acosta Manuel, Arnao Marino B
Departamento de Biología Vegetal (Fisiología Vegetal), Facultad de Biología, Campus Espinardo, Universidad de Murcia, E-30100 Murcia, Spain.
Nahrung. 2002 Oct;46(5):353-6. doi: 10.1002/1521-3803(20020901)46:5<353::AID-FOOD353>3.0.CO;2-B.
The 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)/H2O2/horseradish peroxidase (ABTS/H2O2/HRP) decoloration method permits the evaluation of the antioxidant activity of complex food samples. This method is capable of determining both hydrophilic (in buffered media) and lipophilic (in organic media) antioxidant properties in the same sample. Its application to the study of the antioxidant properties of grapes, one white and two black, has permitted us to establish important differences in the antioxidant activity. The black grapes showed higher antioxidant activity than the white variety. The determination of hydrophilic antioxidant activity (HAA) and lipophilic antioxidant activity (LAA) in the three grapes showed that a minor contribution (4-13%) of total antioxidant activity (TAA) was due to LAA in all cases. The experimentally determined HAA could be approximated using the relative contributions of the calculated values of reference compounds (total phenols as gallic acid and anthocyanins as malvidin).
2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)/过氧化氢/辣根过氧化物酶（ABTS/H₂O₂/HRP）脱色法可用于评估复合食品样品的抗氧化活性。该方法能够在同一样品中测定亲水性（在缓冲介质中）和亲脂性（在有机介质中）抗氧化特性。将其应用于一种白葡萄和两种黑葡萄的抗氧化特性研究，使我们能够确定抗氧化活性的重要差异。黑葡萄的抗氧化活性高于白葡萄品种。对三种葡萄的亲水性抗氧化活性（HAA）和亲脂性抗氧化活性（LAA）的测定表明，在所有情况下，总抗氧化活性（TAA）中LAA的贡献较小（4-13%）。实验测定的HAA可以使用参考化合物（以没食子酸计的总酚和以矢车菊素计的花青素）计算值的相对贡献来近似。
