Xu Yin, McKenna Robert W, Asplund Sheryl L, Kroft Steven H
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75390-9073, USA.
Am J Clin Pathol. 2002 Nov;118(5):758-64. doi: 10.1309/11J6-0U42-VF4E-WA02.
Immunophenotyping of small B-cell neoplasms (SBCNs) may have a critical role in diagnosis. However, there are few data addressing whether the immunophenotypes of SBCNs in bone marrow (BM) and peripheral blood (PB) are representative of those in other tissue sites. We compared the immunophenotypic features of concurrently analyzed lymph node (LN) and BM/PB specimens using multiparameter flow cytometry. Fifty-five SBCNs were identified: 27 follicular lymphomas (FLs), 16 chronic lymphocytic leukemia/small lymphocytic lymphomas (CLL/SLLs), and 12 mantle cell lymphomas (MCLs). Major (presence vs absence) or minor (alteration of intensity) variations in expression of individual antigens between LN and BM/PB were observed in up to 25% of cases within a particular SBCN category. All FLs and CLL/SLLs maintained characteristic immunophenotypes in BM/PB. Potentially misleading variations included 1 case of MCL that failed to express CD5 in BM and likely would have been immunophenotypically misclassified as a marginal zone lymphoma and another MCL that expressed moderate CD23 in PB and would have required additional studies for precise classification. The remaining major and minor variations would not have affected interpretation.
小B细胞肿瘤（SBCNs）的免疫表型分析在诊断中可能起着关键作用。然而，关于骨髓（BM）和外周血（PB）中SBCNs的免疫表型是否代表其他组织部位的免疫表型，相关数据较少。我们使用多参数流式细胞术比较了同时分析的淋巴结（LN）与BM/PB标本的免疫表型特征。共鉴定出55例SBCNs：27例滤泡性淋巴瘤（FLs）、16例慢性淋巴细胞白血病/小淋巴细胞淋巴瘤（CLL/SLLs）和12例套细胞淋巴瘤（MCLs）。在特定SBCN类别中，高达25%的病例在LN与BM/PB之间观察到个别抗原表达的主要（存在与否）或次要（强度改变）差异。所有FLs和CLL/SLLs在BM/PB中均维持特征性免疫表型。可能产生误导的差异包括1例MCL在BM中未表达CD5，免疫表型上可能会被错误分类为边缘区淋巴瘤；另一例MCL在PB中表达中等水平的CD23，需要进一步研究以进行精确分类。其余的主要和次要差异不会影响诊断解读。
