Byrne Liam, Lau King Tong, Diamond Dermot
National Centre for Sensor Research, Dublin City University, Dublin, Ireland.
Analyst. 2002 Oct;127(10):1338-41. doi: 10.1039/b206149j.
The release of amines from decomposing fish such as trimethylamine (TMA), dimethylamine (DMA) and ammonia, collectively known as total volatile basic nitrogen (TVB-N), are in high enough concentrations in headspace to be monitored by a colour change in a pH-sensitive sensor. A method developed here uses a pH indicator dye physically trapped in a cellulose polymer film to respond to the headspace TVB-N released from selected fish species during spoilage. Two species were selected for analysis on the basis of economic importance and the levels of volatile amines released were followed with time using uv/vis reflectance spectroscopic measurements. The results show that there is a significant increase in the TVB-N content in the headspace of fish samples after an incubation period of 8-12 h for cod and 12-15 h for orange roughy.
鱼类分解时释放出的胺类物质，如三甲胺（TMA）、二甲胺（DMA）和氨，统称为总挥发性碱性氮（TVB-N），其在顶空中的浓度足够高，可通过pH敏感传感器的颜色变化进行监测。本文开发的一种方法是使用物理捕获在纤维素聚合物膜中的pH指示剂染料，来响应特定鱼类在腐败过程中释放到顶空中的TVB-N。根据经济重要性选择了两个物种，并使用紫外/可见反射光谱测量法，随时间跟踪释放的挥发性胺类物质的含量。结果表明，鳕鱼在孵育8 - 12小时、红鱼在孵育12 - 15小时后，鱼样本顶空中的TVB-N含量显著增加。
