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[棉花种子中7S球蛋白(棉豆球蛋白-1)的非同源亚基]

[Non-identical subunits of 7S globulin (gossipulin-1) from cotton seeds].

作者信息

Ibragimov A P, Yunuskhanov S, Tursunbaev P, Tuichiev A B, Abdurachmanova B

出版信息

Biokhimiia. 1975 May-Jun;40(3):605-10.

PMID:1243097
Abstract

7S globulin is isolated from cotton seeds and is designated as "Gossipulin-1". Three different amino acids (glycine, aspartic acid and leucine) are found to follow the N-terminal amino acid which is indicative of a non-identity of its subunits. Gossipulin-1 dissociated in 0.1 M HCl, depending on the time of incubation, into 3.87, 2.39 and 1.35 subunits. It had a sedimentation constant of 0.37S in alkaline medium and of 1.24S on 8 M urea. A sedimentation peak of 1.1S was discovered in reduced and alkylated Gossipulin-1.

摘要

7S球蛋白是从棉籽中分离出来的,被命名为“棉籽球蛋白-1”。发现三种不同的氨基酸(甘氨酸、天冬氨酸和亮氨酸)紧跟在N端氨基酸之后,这表明其亚基具有非同一性。棉籽球蛋白-1在0.1 M盐酸中解离,根据孵育时间不同,可解离为3.87、2.39和1.35个亚基。它在碱性介质中的沉降常数为0.37S,在8 M尿素中为1.24S。在还原和烷基化的棉籽球蛋白-1中发现了一个1.1S的沉降峰。

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