Brecher Arthur S, Hommema Eric L
Department of Chemistry, Bowling Green State University, OH 43403, USA.
Can J Physiol Pharmacol. 2002 Sep;80(9):879-86. doi: 10.1139/y02-111.
The comparative effects of glycosaminoglycans and acetaldehyde (AcH)--glycosaminoglycan (GAG) mixtures upon Factor Xa- (FXa) and Factor X-deficient plasma (FXDP) have been studied by activated partial thromboplastin time (APTT) studies. Heparin at 0.025, 0.030, 0.04, and 0.05 U statistically prolonged the APTT when pre-incubated with FXa at 37 degrees C for 3 min prior to addition to FXDP and subsequent addition of Ca2+. Upon addition of 0.25, 0.375, and 0.5 microg heparin-6000 (H6k) to FXa, significant increases in APTT were observed. Similarly, profound increases in APTT were observed when 0.5, 0.75, and 1.0 microg heparin-3000 (H3k) was added to FXa. The chondroitin sulfates (CSA, CSB, CSC) had far less impact upon APTT with the FXa-FXDP system. In examining the effects of AcH-GAG mixtures upon the clotting factor, it was observed that 44.3 and 443 mM AcH synergistically prolonged the APTT in a statistically significant manner regardless of the order of premixing the three components. Hence, AcH may play a role in prolonging APTT in alcoholics. It synergistically prolonged APTT in concert with GAGs and FXa at the AcH levels used in this study. The effect of the GAGs upon FXDP is far less than its effect upon FXa.
通过活化部分凝血活酶时间（APTT）研究，对糖胺聚糖和乙醛（AcH）-糖胺聚糖（GAG）混合物对凝血因子Xa（FXa）和缺乏凝血因子X的血浆（FXDP）的比较作用进行了研究。在37℃下与FXa预孵育3分钟，然后加入FXDP并随后加入Ca2+，0.025、0.030、0.04和0.05单位的肝素在统计学上延长了APTT。向FXa中加入0.25、0.375和0.5微克肝素-6000（H6k）后，观察到APTT显著增加。同样，当向FXa中加入0.5、0.75和1.0微克肝素-3000（H3k）时，观察到APTT大幅增加。硫酸软骨素（CSA、CSB、CSC）对FXa-FXDP系统的APTT影响要小得多。在研究AcH-GAG混合物对凝血因子的影响时，观察到44.3和443 mM的AcH无论三种成分预混合的顺序如何，均以统计学上显著的方式协同延长APTT。因此，AcH可能在延长酗酒者的APTT中起作用。在本研究使用的AcH水平下，它与GAG和FXa协同延长APTT。GAG对FXDP的作用远小于其对FXa的作用。
