秀丽隐杆线虫中的中心体成熟和有丝分裂纺锤体组装需要SPD-5，一种具有多个卷曲螺旋结构域的蛋白质。

Centrosome maturation and mitotic spindle assembly in C. elegans require SPD-5, a protein with multiple coiled-coil domains.

作者信息

Hamill Danielle R, Severson Aaron F, Carter J Clayton, Bowerman Bruce

机构信息

Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.

出版信息

Dev Cell. 2002 Nov;3(5):673-84. doi: 10.1016/s1534-5807(02)00327-1.

DOI:10.1016/s1534-5807(02)00327-1

PMID:12431374

Abstract

The maternally expressed C. elegans gene spd-5 encodes a centrosomal protein with multiple coiled-coil domains. During mitosis in mutants with reduced levels of SPD-5, microtubules assemble but radiate from condensed chromosomes without forming a spindle, and mitosis fails. SPD-5 is required for the centrosomal localization of gamma-tubulin, XMAP-215, and Aurora A kinase family members, but SPD-5 accumulates at centrosomes in mutants lacking these proteins. Furthermore, SPD-5 interacts genetically with a dynein heavy chain. We propose that SPD-5, along with dynein, is required for centrosome maturation and mitotic spindle assembly.

摘要

母源表达的秀丽隐杆线虫基因spd-5编码一种具有多个卷曲螺旋结构域的中心体蛋白。在SPD-5水平降低的突变体有丝分裂过程中，微管会组装，但从浓缩染色体发出而不形成纺锤体，导致有丝分裂失败。γ-微管蛋白、XMAP-215和极光激酶A家族成员的中心体定位需要SPD-5，但在缺乏这些蛋白的突变体中，SPD-5会在中心体积累。此外，SPD-5与动力蛋白重链存在遗传相互作用。我们提出，SPD-5与动力蛋白一起，是中心体成熟和有丝分裂纺锤体组装所必需的。