γ-微管蛋白37C和γ-微管蛋白环复合物蛋白75在果蝇卵子发生过程中对双尾RNA定位至关重要。

Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis.

作者信息

Schnorrer Frank, Luschnig Stefan, Koch Iris, Nüsslein-Volhard Christiane

机构信息

Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Spemannstr. 35, 72076, Tübingen, Germany.

出版信息

Dev Cell. 2002 Nov;3(5):685-96. doi: 10.1016/s1534-5807(02)00301-5.

DOI:10.1016/s1534-5807(02)00301-5

PMID:12431375

Abstract

bicoid (bcd) RNA localization requires the activity of exuperantia and swallow at sequential steps of oogenesis and is microtubule dependent. In a genetic screen, we identified two novel genes essential for bcd RNA localization. They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components. Mutations in these genes specifically affect bcd RNA localization, whereas other microtubule-dependent processes during oogenesis are not impaired. This provides direct evidence that a subset of microtubules organized by the gamma-tubulin ring complex is essential for localization of bcd RNA. At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte.

摘要

双尾（bcd）RNA定位在卵子发生的连续步骤中需要exuperantia和swallow的活性，并且依赖于微管。在一项遗传筛选中，我们鉴定出两个对bcd RNA定位至关重要的新基因。它们编码母体γ-微管蛋白37C（γTub37C）和γ-微管蛋白环复合物蛋白75（Dgrip75），二者均为γ-微管蛋白环复合物组分。这些基因中的突变特异性地影响bcd RNA定位，而卵子发生过程中的其他微管依赖过程未受损害。这提供了直接证据，表明由γ-微管蛋白环复合物组织的一部分微管对于bcd RNA的定位至关重要。在第10b阶段，我们发现γTub37C和Dgrip75在前部集中，并提出在卵母细胞的前极形成一个微管组织中心。