II类内含子：具有模块化结构和多种催化功能的高度特异性内切核酸酶。

Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.

作者信息

Fedorova Olga, Su Linhui Julie, Pyle Anna Marie

机构信息

Department of Biochemistry and Molecular Biophysics, 630 West 168 Street, Box 36, Columbia University, New York, NY 10032, USA.

出版信息

Methods. 2002 Nov;28(3):323-35. doi: 10.1016/s1046-2023(02)00239-6.

DOI:10.1016/s1046-2023(02)00239-6

PMID:12431436

Abstract

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.

摘要

II 组内含子是具有一系列显著反应的大型催化 RNA。本文介绍了用于开发一组动力学框架的构建体设计和方案，这些动力学框架用于研究 II 组内含子及其衍生的核酶的结构和反应机制。此外，我们还讨论了这些系统在 ai5γ II 组内含子结构/功能分析中的应用。