Choi Jung-Kap, Yoo Gyurng-Soo
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, Korea.
Arch Pharm Res. 2002 Oct;25(5):704-8. doi: 10.1007/BF02976948.
A fast and sensitive protein staining method in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using both an acidic dye, Coomassie Brilliant Blue R-250 (CBBR) and a basic dye, Neutral Red (NR) is described. It is based on a counter ion-dye staining technique that employs oppositely charged two dyes to form an ion-pair complex. The selective binding of the free dye molecules to proteins in an acidic solution enhances the staining effect of CBBR on protein bands, and also reduces gel background. It is a rapid staining procedure, involving fixing and staining steps with short destaining that are completed in about 1 h. As the result, it showed two to fourfold increase in sensitivity comparing with CBBR staining. The stained protein bands can be visualized at the same time of staining.
描述了一种在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中使用酸性染料考马斯亮蓝R-250（CBBR）和碱性染料中性红（NR）的快速灵敏蛋白质染色方法。它基于一种反离子染料染色技术，该技术使用带相反电荷的两种染料形成离子对复合物。在酸性溶液中，游离染料分子与蛋白质的选择性结合增强了CBBR对蛋白条带的染色效果，同时也降低了凝胶背景。这是一种快速染色程序，包括固定和染色步骤以及短时间脱色，大约1小时即可完成。结果表明，与CBBR染色相比，其灵敏度提高了两到四倍。染色的蛋白条带在染色的同时即可观察到。
