Inés Toral M, Pope Stefanie, Quintanilla Silvia, Richter Pablo
Department of Chemistry, University of Chile, P O Box 653, Santiago, Chile.
Int J Pharm. 2002 Dec 5;249(1-2):117-26. doi: 10.1016/s0378-5173(02)00482-9.
This work presents a simple and fast method for the simultaneous determination of amiloride and furosemide by digital derivative spectrophotometry. HCl 1 x 10(-2) mol/l dissolved in ethanol was used as solvent and to extract drugs from formulations. Subsequently the samples were evaluated directly by first digital derivative spectrophotometry, using a smoothing factor of 8 and scale factor of 1 x 10(-4). The simultaneous determination of furosemide and amiloride can be carried out at 241.4 and 343.6 nm, respectively. In both cases, the zero crossing approach was used. When both compounds are present together in a sample, it is possible to quantify one in the presence of the other, without mutual interference. The determination range was found to be of 6.9 x 10(-8) to 16 x 10(-5) and 6.8 x 10(-8) to 8 x 10(-5) mol/l, for amiloride and furosemide, respectively. A good level of repeatability (RSD) of 0.9 and 0.6% was observed for amiloride and furosemide, respectively. The ingredients commonly found in commercial pharmaceutical formulations do not interfere. The proposed method was applied to the determination of these drugs in pharmaceutical formulations.
本研究提出了一种通过数字导数分光光度法同时测定阿米洛利和呋塞米的简单快速方法。将溶解于乙醇中的1×10⁻² mol/l盐酸用作溶剂，从制剂中提取药物。随后，使用平滑因子8和比例因子1×10⁻⁴，通过一阶数字导数分光光度法直接对样品进行评估。呋塞米和阿米洛利的同时测定可分别在241.4和343.6 nm处进行。在这两种情况下，均采用零交叉法。当两种化合物同时存在于样品中时，可以在一种化合物存在的情况下对另一种进行定量，而不会相互干扰。阿米洛利和呋塞米的测定范围分别为6.9×10⁻⁸至16×10⁻⁵和6.8×10⁻⁸至8×10⁻⁵ mol/l。阿米洛利和呋塞米的重复性良好（相对标准偏差），分别为0.9%和0.6%。商业药物制剂中常见的成分不会产生干扰。所提出的方法应用于药物制剂中这些药物的测定。
