[间接原位巢式聚合酶链反应检测肺癌组织中的结核分枝杆菌]
[Detection of Mycobacterium tuberculosis in lung cancer tissue by indirect in situ nested PCR].
作者信息
Song Lan-Ying, Yan Wen-Sheng, Zhao Tong
机构信息
Department of Pathology, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):992-3.
OBJECTIVE
To localize Mycobacterium tuberculosis DNA (TB-DNA) in lung cancer tissue and to investigate the possible relationship of this bacterial infection with the development of lung cancer.
METHODS
A sensitive and specific indirect in situ nested PCR (ISNPCR) was used to identify and localize TB-DNA in 15 formalin fixed paraffin-embedded lung cancer tissue specimens, which had been demonstrated to be positive for TB-DNA by conventional PCR.
RESULTS
Positive granules of TB-DNA in brown color was found mainly in the cytoplasm of the alveolar epithelial cells, pulmonary macrophages, inflammatory cells and a few tumor cells within lung cancer tissues.
CONCLUSION
Mycobacterium tuberculosis infection may play a role in the pathogenesis of lung cancer.
目的
在肺癌组织中定位结核分枝杆菌DNA（TB-DNA），并探讨这种细菌感染与肺癌发生之间可能存在的关系。
方法
采用灵敏且特异的间接原位巢式聚合酶链反应（ISNPCR），对15例经福尔马林固定、石蜡包埋的肺癌组织标本中的TB-DNA进行鉴定和定位，这些标本经传统聚合酶链反应已证实TB-DNA呈阳性。
结果
在肺癌组织中，棕色的TB-DNA阳性颗粒主要见于肺泡上皮细胞、肺巨噬细胞、炎性细胞及少数肿瘤细胞的胞质内。
结论
结核分枝杆菌感染可能在肺癌发病机制中起作用。
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