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[含多聚（A）尾的大肠杆菌信使核糖核酸互补脱氧核糖核酸文库的构建与鉴定]

[Construction and identification of cDNA library of E.coli mRNA with poly(A) tracts].

作者信息

Hu Zi-You, Ma Wen-Li, Wu Qing-Hua, Zhang Bao, Song Yan-Bin, Guo Qiu-Ye, Zheng Wen-Ling

机构信息

Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):1005-7.

PMID:12433631
Abstract

OBJECTIVE

To construct and identify the cDNA library of E.coli mRNA with poly(A) tracts.

METHODS

The cDNA library of E.coli was constructed by restriction display-PCR (RD-PCR) technique, followed by sequencing and bioinformatics analyses.

RESULTS

cDNA library of E.coli mRNA with poly(A) tracts was successfully constructed, and 66 gene fragments were sequenced.

CONCLUSION

The constructed cDNA library of E.coli mRNA with poly(A) tracts contains a low rate of repetition and is of high quality.

摘要

目的

构建并鉴定含poly(A)尾的大肠杆菌mRNA的cDNA文库。

方法

采用限制性展示PCR（RD-PCR）技术构建大肠杆菌cDNA文库，随后进行测序及生物信息学分析。

结果

成功构建了含poly(A)尾的大肠杆菌mRNA的cDNA文库，并对66个基因片段进行了测序。

结论

构建的含poly(A)尾的大肠杆菌mRNA的cDNA文库重复率低，质量较高。

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Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):1005-7.
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