Chen Gang, Lin Li-Xiang, Zhuang Wei-Te, Yao Jin, Liang Ji-Xin, Huang Hui-Bin, Lin Fan, Lin Jian-Li
Department of Endocrinology, Fujian Provincial Hospital, Fuzhou 350001, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):1009-10.
To study the pathogenesis of diabetic cardiomyopathy using DNA microarray.
Experimental rats were grouped into a diabetic cardiomyopathy and a normal control group. The cDNA probes were prepared by labeling the mRNA extracted from normal tissue and tissue of diabetic cardiomyopathy with Cy3-dUTP and Cy5-dUTP respectively through reverse transcription. DNA microarray were constructed by spotting PCR products of 4 000 rat cDNAs onto a specially treated glass slides, and were then hybridized against the cDNA probes followed by fluorescent signals scanning.
The expression of energy metabolism-related genes were lower in the tissues of diabetic cardiomyopathy than in normal tissue.
The energy metabolism disorder may play an important role in the pathogenesis of diabetic cardiomyopathy.
