Palomo Ivan, Pereira Jaime, Alarcon Marcelo, Larrain Ana Maria, Pinochet Carmen, Vasquez Marcela, Velez Maria T, Leon M, Espinola Ricardo, Pierangeli Silvia
Department of Clinical Biochemistry and Immunohematology, School of Health Sciences, University of Talca.
J Lab Clin Med. 2002 Nov;140(5):336-41. doi: 10.1067/mlc.2002.128467.
Antiphospholipid antibodies (aPLs) are a heterogeneous family of antibodies found in autoimmune disorders, infectious diseases, and other situations. The presence of different aPLs has been associated with various clinical manifestations of the antiphospholipid syndrome (APS). The objective of this study was to investigate the prevalence of aPLs in a group of 90 Chilean patients with systemic lupus erytematosus (SLE) and 90 healthy controls. We measured anticardiolipin antibodies (aCLs), antiphosphatidylserine antibodies (aPSs), anti-beta(2) glycoprotein I antibodies (anti-beta(2)GPIs), and antiprothrombin antibodies (aPTs) with an enzyme-linked immunosorbent technique using "in-house" assays. Fifty-four of 90 SLE patients (60.0%) had some type of aPL. Forty of 90 (44.4%) were positive for aCLs, 9 of 61 (14.8%) had aPSs, 21 of 90 (23.3%) had anti-beta(2)GPIs, and 18 of 90 (20.0%) had aPTs. In the control group, prevalences were as follows: aCLs, 3.3%; aPSs, 1.1%; anti-beta(2)GPIs, 1.1%; aPTs, 2.2%. In most cases, values were in the low-positive range. Of all aPL detected, 29.5% was of the IgG isotype, 37.5% IgM, and 33.0% IgA. We observed a correlation between aCLs and aPSs and of these antibodies with anti-beta(2)GPIs and aPTs but not between anti-beta(2)GPIs and aPTs. Our results show a high prevalence of aPLs in SLE patients. An association between different specificities and isotypes of aPLs was also observed.
抗磷脂抗体（aPLs）是一类异质性抗体家族，见于自身免疫性疾病、感染性疾病及其他情况。不同抗磷脂抗体的存在与抗磷脂综合征（APS）的多种临床表现相关。本研究的目的是调查90例智利系统性红斑狼疮（SLE）患者及90例健康对照者中抗磷脂抗体的患病率。我们采用“内部”检测的酶联免疫吸附技术检测了抗心磷脂抗体（aCLs）、抗磷脂酰丝氨酸抗体（aPSs）、抗β2糖蛋白I抗体（抗β2GPIs）和抗凝血酶原抗体（aPTs）。90例SLE患者中有54例（60.0%）存在某种类型的抗磷脂抗体。90例中有40例（44.4%）aCLs阳性，61例中有9例（14.8%）aPSs阳性，90例中有21例（23.3%）抗β2GPIs阳性，90例中有18例（20.0%）aPTs阳性。在对照组中，患病率如下：aCLs为3.3%；aPSs为1.1%；抗β2GPIs为1.1%；aPTs为2.2%。在大多数情况下，数值处于低阳性范围。在所有检测到的抗磷脂抗体中，29.5%为IgG同种型，37.5%为IgM，33.0%为IgA。我们观察到aCLs与aPSs之间以及这些抗体与抗β2GPIs和aPTs之间存在相关性，但抗β2GPIs与aPTs之间无相关性。我们的结果显示SLE患者中抗磷脂抗体的患病率较高。还观察到抗磷脂抗体不同特异性和同种型之间存在关联。
