Swan Jeremy S, Arango Maria E, Carothers Carraway Coralie A, Carraway Kermit L
Department of Cell Biology and Anatomy, University of Miami School of Medicine, Miami, FL, USA.
Curr Eye Res. 2002 May;24(5):397-402. doi: 10.1076/ceyr.24.5.397.8521.
To show the presence and localization of type 1 growth factor receptors (ErbB2, ErbB3 and ErbB4) in rat corneal and conjunctival epithelia and investigate the association of ErbB2 with its intramembrane ligand Muc4.
Methacarn-fixed, paraffin-embedded sections of corneas and eyelids from female adult rats were immunocytochemically stained using antibodies against the ErbB receptors and Muc4. Sequential immunoprecipitation and immunoblot analyses were performed on epithelial lysates to investigate the presence of a complex of Muc4 and ErbB2 in corneal and conjunctival epithelia.
Immunocytochemical staining demonstrated the presence of ErbB2, ErbB3 and ErbB4 growth factor receptors throughout the rat corneal and conjunctival epithelia. Co-immunoprecipitation of the epithelial lysates demonstrated that Muc4 and ErbB2 are present as a complex.
The three type 1 growth factor receptors (ErbB2, ErbB3 and ErbB4) are present in the rat corneal and conjunctival epithelia, and ErbB2 is at least partly associated with Muc4. This demonstration of the presence and localization of these three type 1 growth factor receptors may help in understanding how these receptors contribute to ocular epithelial behavior and functions.
显示1型生长因子受体（ErbB2、ErbB3和ErbB4）在大鼠角膜和结膜上皮中的存在及定位，并研究ErbB2与其膜内配体Muc4的关联。
使用抗ErbB受体和Muc4的抗体对成年雌性大鼠的角膜和眼睑经甲醇-氯仿固定、石蜡包埋的切片进行免疫细胞化学染色。对上皮裂解物进行连续免疫沉淀和免疫印迹分析，以研究角膜和结膜上皮中Muc4和ErbB2复合物的存在情况。
免疫细胞化学染色显示，ErbB2、ErbB3和ErbB4生长因子受体存在于整个大鼠角膜和结膜上皮中。上皮裂解物的共免疫沉淀表明，Muc4和ErbB2以复合物形式存在。
三种1型生长因子受体（ErbB2、ErbB3和ErbB4）存在于大鼠角膜和结膜上皮中，且ErbB2至少部分与Muc4相关。这三种1型生长因子受体的存在及定位的证明可能有助于理解这些受体如何影响眼表上皮的行为和功能。
