Georgiades Pantelis, Ogilvy Sarah, Duval Hélène, Licence Diana R, Charnock-Jones D Stephen, Smith Stephen K, Print Cristin G
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK.
Genesis. 2002 Dec;34(4):251-6. doi: 10.1002/gene.10161.
Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is expressed under control of murine vav gene regulatory elements. To assess their usefulness, vavCre transgenic mice were bred with R26R mice, which express a lacZ reporter gene only in cells where Cre-mediated recombination has occurred. VavCre/R26R double-heterozygous offspring were analyzed by beta-galactosidase histochemistry and flow cytometry. VavCre-mediated recombination occurred in most hematopoietic cells of all hematopoietic organs, including the hematopoietic progenitor-rich bone marrow. Recombination also occurred in most endothelial and germ cells, but only rarely in other cell types. The recombination in both hematopoietic and endothelial lineages may partly reflect their putative shared ontogeny and provides a unique tool for simultaneous pan-hematopoietic and endothelial mutagenesis.
Cre转基因小鼠可用于删除特定体细胞组织中位于loxP位点两侧的基因序列。我们已培育出vavCre转基因小鼠，可用于在成年造血细胞和内皮细胞中特异性地使基因失活。在这些动物中，Cre转基因在小鼠vav基因调控元件的控制下表达。为评估其效用，将vavCre转基因小鼠与R26R小鼠杂交，后者仅在发生Cre介导的重组的细胞中表达lacZ报告基因。通过β-半乳糖苷酶组织化学和流式细胞术对VavCre/R26R双杂合子后代进行分析。VavCre介导的重组发生在所有造血器官的大多数造血细胞中，包括富含造血祖细胞的骨髓。重组也发生在大多数内皮细胞和生殖细胞中，但在其他细胞类型中很少发生。造血和内皮谱系中的重组可能部分反映了它们假定的共同个体发生，并为同时进行全造血和内皮诱变提供了一个独特的工具。
