Xu Zhen, Wang Bai-Ren, Ding Yu-qiang, Kuang Fang, Wang Xi, Duan Xiao-Li, Jiao Xi-Ying, Ju Gong
Institute of Neurosciences, The Fourth Military Medical University, Xi'an, 710032, PR China.
Neurosci Lett. 2002 Dec 13;334(2):103-6. doi: 10.1016/s0304-3940(02)01113-8.
Phosphorylated extracellular signal-regulated kinases1/2 (pERK1/2) -like immunoreactivity (LI) was enhanced in the neurons of the nucleus of the solitary tract (NTS) and dorsal motor nucleus of the vagus nerve (DMV) 8 min after an intraperitoneal injection of acetic acid in the mouse; the enhancement in pERK1/2-LI was suppressed after vagotomy. With immunofluorescent double labeling technique, the co-localization of acetic acid-induced pERK1/2 and tyrosine hydroxylase-LIs was observed in some of the NTS and DMV neurons. These results suggest that ERK1/2 signal-transducting pathway is involved in neuronal activities in NTS and DMV which are induced by vagus-conveyed nociceptive visceral information, and that some of these pERK1/2- immunoreactive neurons in NTS and DMV are catecholaminergic.
腹腔注射醋酸8分钟后，小鼠孤束核（NTS）和迷走神经背运动核（DMV）神经元中磷酸化细胞外信号调节激酶1/2（pERK1/2）样免疫反应性（LI）增强；迷走神经切断术后，pERK1/2-LI的增强受到抑制。采用免疫荧光双标记技术，在部分NTS和DMV神经元中观察到醋酸诱导的pERK1/2与酪氨酸羟化酶-LI的共定位。这些结果表明，ERK1/2信号转导通路参与了由迷走神经传递的伤害性内脏信息诱导的NTS和DMV神经元活动，并且NTS和DMV中一些pERK1/2免疫反应性神经元是儿茶酚胺能的。
