Yonehara Keisuke, Suzuki Masatoshi, Yamanouchi Keitaro, Nishihara Masugi
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Tokyo 113-8657, Japan.
Neurosci Lett. 2002 Dec 13;334(2):107-10. doi: 10.1016/s0304-3940(02)01114-x.
Our previous research using cDNA microarray analysis demonstrated that female rats displayed a higher p130 mRNA level than males in the hypothalamus at postnatal day (PN) 5. In the present study, it was shown that at PN3 males had a significantly elevated mRNA level over females, whereas at PN7 females displayed a higher expression level using a real-time reverse transcription-polymerase chain reaction. In situ hybridization analysis indicated relatively strong p130 mRNA signals in the ventromedial nucleus and the arcuate nucleus in the neonatal hypothalamus. Subcutaneous injection of 5alpha-dihydrotestosterone as well as testosterone propionate to PN2 neonatal rats significantly increased p130 gene expression at PN3, whereas estradiol benzoate did not have a significant effect. These results suggest that expression of the p130 gene in the neonatal rat hypothalamus is responsive to androgens and may be involved in sexual differentiation of the brain.
我们先前利用cDNA微阵列分析的研究表明，出生后第5天（PN5）时，雌性大鼠下丘脑的p130 mRNA水平高于雄性。在本研究中，结果显示，出生后第3天（PN3）时雄性大鼠的mRNA水平显著高于雌性，而出生后第7天（PN7）时，通过实时逆转录-聚合酶链反应检测发现雌性大鼠的表达水平更高。原位杂交分析表明，新生大鼠下丘脑腹内侧核和弓状核中p130 mRNA信号相对较强。给出生后第2天（PN2）的新生大鼠皮下注射5α-二氢睾酮以及丙酸睾酮，可显著增加出生后第3天（PN3）时p130基因的表达，而苯甲酸雌二醇则没有显著影响。这些结果表明，新生大鼠下丘脑p130基因的表达对雄激素有反应，可能参与大脑的性别分化。
