Régnacq Matthieu, Ferreira Thierry, Puard Julien, Bergès Thierry
Laboratoire de Génétique de la Levure, CNRS-UMR 6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Cedex, Poitiers, France.
FEMS Microbiol Lett. 2002 Nov 5;216(2):165-70. doi: 10.1111/j.1574-6968.2002.tb11431.x.
SUT1 constitutive expression in aerobiosis suppressed the ts phenotype of the sec14-1 mutation, restored growth of the sec14-null mutant and corrected the translocation defect of the vacuolar carboxypeptidase Y. Therefore SUT1 was shown to be a novel potent sec14-1 suppressor. Further, the hypoxic gene CSR1 (YLR380W), a Sec14 homolog, was upregulated upon SUT1 constitutive expression. In addition, SUT1 effects on both sec14-1 suppression and on free sterol composition were abolished in a csr1-null background, showing that this gene acts downstream of SUT1.
在需氧条件下SUT1的组成型表达抑制了sec14 - 1突变的温度敏感表型，恢复了sec14缺失突变体的生长，并纠正了液泡羧肽酶Y的转运缺陷。因此，SUT1被证明是一种新型的强效sec14 - 1抑制因子。此外，低氧基因CSR1（YLR380W），一种Sec14同源物，在SUT1组成型表达时上调。另外，在csr1缺失背景下，SUT1对sec14 - 1抑制和游离甾醇组成的影响均被消除，表明该基因在SUT1下游起作用。
