Kawasaki Yoko, Sugimoto Naoki, Sato Kyoko, Yamazaki Takeshi, Ishiwata Hajimu, Maitani Tamio
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Shokuhin Eiseigaku Zasshi. 2002 Aug;43(4):191-5. doi: 10.3358/shokueishi.43.191.
An unknown red pigment was purified from an apple syrup product imported from Canada, using a DIAION HP-20 column with methanol as the eluent. By spectroscopic means and chemical synthesis, the isolated pigment was identified as 4-aminocarminic acid, which is the major pigment of acid-stable carmine (a red colorant illegal in Japan). In addition, HPLC and TLC methods were proposed to detect this illegal colorant. While the color of carminic acid changed from yellow to red in the pH range of McIlvaine buffer (3.0-7.0), the color of 4-aminocarminic acid was always red, and also the ultraviolet/visible (UV/Vis) spectra did not change. These characteristics are useful to distinguish 4-aminocarminic acid from carminic acid.
从加拿大进口的苹果糖浆产品中，使用以甲醇为洗脱剂的DIAION HP - 20柱纯化出一种未知红色色素。通过光谱手段和化学合成，鉴定出分离出的色素为4 - 氨基胭脂红酸，它是酸稳定胭脂红（一种在日本非法的红色素）的主要色素。此外，还提出了高效液相色谱法（HPLC）和薄层色谱法（TLC）来检测这种非法色素。在麦克利文缓冲液（pH 3.0 - 7.0）范围内，胭脂红酸的颜色从黄色变为红色，而4 - 氨基胭脂红酸的颜色始终为红色，并且紫外/可见（UV/Vis）光谱也没有变化。这些特性有助于区分4 - 氨基胭脂红酸和胭脂红酸。
