Separation of biosynthetic polyunsaturated fatty acid (PUFA) with supercritical fluid.

The separation of biosynthetic polyunsaturated fatty acid (PUFA) was undertaken with supercritical fluid. The polyunsaturated fatty acid was synthesised by WL-1021, which is a strain of marine bacterium isolated from marine fish. The polyunsaturated fatty acid can be very efficiently extracted from WL-1021 that has the characteristics of rapid growth in the artificial medium. The PUFA in WL-1021 has been successfully separated by supercritical fluid extraction (SFE). The SFE process parameters including pressure, temperature and culture temperature variations have been measured by High Performance Liquid Chromatography (HPLC), GC/FT-IR and GC/MS.