Grote Monika, Stumvoll Sabine, Reichelt Rudolf, Lidholm Jonas, Rudolf Valenta
Institute of Medical Physics and Biophysics, University of Münster, Germany.
Biol Chem. 2002 Sep;383(9):1441-5. doi: 10.1515/BC.2002.163.
Group 4 grass pollen allergens represent 60 kDa glycoproteins recognized by 70% of patients sensitive to these pollens. An antiserum against purified Phl p 4 from timothy grass pollen was used to investigate various pollens, fruits, and vegetables for Phl p 4-related allergens by immunogold electron microscopy. In timothy grass, mugwort, and birch pollens, allergens were located in the wall, and in timothy grass and birch pollens additionally in the cytoplasm. In peanut, apple, celery root, and carrot root, only cytoplasmic areas were labeled. Group 4-related allergens thus occur in pollens of unrelated plants and in plant food and may therefore contribute to crossreactivities in patients allergic to various pollens and plant food.
第4组草花粉过敏原是分子量为60 kDa的糖蛋白，70%对这些花粉敏感的患者可识别此类蛋白。利用抗纯化梯牧草花粉Phl p 4的抗血清，通过免疫金电子显微镜研究了各种花粉、水果和蔬菜中与Phl p 4相关的过敏原。在梯牧草、艾蒿和桦树花粉中，过敏原位于细胞壁，在梯牧草和桦树花粉中还存在于细胞质中。在花生、苹果、芹菜根和胡萝卜根中，仅细胞质区域有标记。因此，第4组相关过敏原存在于不相关植物的花粉和植物性食物中，可能会导致对各种花粉和植物性食物过敏的患者出现交叉反应。
