Bazylak Grzegorz, Nagels Luc J
Department of Chemistry, University of Antwerp (RUCA), Belgium.
J Chromatogr A. 2002 Oct 11;973(1-2):85-96. doi: 10.1016/s0021-9673(02)01190-1.
Potentiometric detection with poly(vinyl chloride) (PVC) based liquid membrane electrode coatings is presented for a series of 18 exogenic beta-adrenergic substances in cation-exchange-HPLC and RP-HPLC systems. In both types of HPLC modes employing hybrid polymer-silica packings we observed that use of tetrakis(p-chlorophenyl)borate (TCPB) containing electrodes yielded limits of detection (DL) down to 10(-7)-10(-8) M (injected concentrations). The use of eluents with high concentrations of acetonitrile (up to 55%) yielded detection limits down to 10(-9) M (injected concentrations). A quantitative structure-potentiometric response activity relationship (QSAR) was developed for the set of beta-adrenergic substances and for a set of PVC-based electrodes using TCPB alone or in admixture with trioctylated alpha-cyclodextrin, dibenzo-18-crown-6, or calix[6]arene hexaethylester. A multiple linear regression model based on a computationally derived set of 14 molecular descriptors allowed prediction of the detection limits of beta-adrenergic substances and other amine substances from their molecular structure.
本文介绍了基于聚氯乙烯（PVC）液膜电极涂层的电位检测方法，用于阳离子交换高效液相色谱（HPLC）和反相高效液相色谱（RP-HPLC）系统中的一系列18种外源性β-肾上腺素能物质。在采用混合聚合物-硅胶填料的两种HPLC模式中，我们观察到使用含四（对氯苯基）硼酸盐（TCPB）的电极时，检测限（DL）低至10^(-7)-10^(-8) M（进样浓度）。使用高浓度乙腈（高达55%）的洗脱液时，检测限低至10^(-9) M（进样浓度）。针对β-肾上腺素能物质组以及单独使用TCPB或与三辛基化α-环糊精、二苯并-18-冠-6或杯[6]芳烃六乙酯混合的一组基于PVC的电极，建立了定量结构-电位响应活性关系（QSAR）。基于一组通过计算得出的14个分子描述符的多元线性回归模型，能够从β-肾上腺素能物质和其他胺类物质的分子结构预测其检测限。
