Gaetani Gian Franco, Rapezzi Davide, Mangerini Rosa, Racchi Omar, Rolfo Michela, Ferraris Anna Maria
Divisione di Ematologia Oncologica, Istituto Scientifico Tumori, Dipartimento di Oncologia, Biologia e Genetica, Università of Genova, Genova, Italy.
Br J Haematol. 2002 Dec;119(3):833-8. doi: 10.1046/j.1365-2141.2002.03865.x.
Methylene blue (MB) is a powerful reducing agent that is widely used in clinical practice as well as for metabolic studies of the erythrocyte. We have investigated the role of catalase as a specific enzyme for the removal of hydrogen peroxide by measuring the in vitro effects of MB on human red cells. In the presence of MB, catalase underwent inactivation even with the co-existence of active generation of NADPH, leaving the glutathione concentration unaffected. The data obtained in the present investigation show, using a different tool (MB), that catalase is the active enzyme in H2O2 detoxification and that its integrity is largely dependent on an adequate generation of NADPH.
亚甲蓝（MB）是一种强还原剂，广泛应用于临床实践以及红细胞的代谢研究。我们通过测量MB对人红细胞的体外作用，研究了过氧化氢酶作为去除过氧化氢的特异性酶的作用。在MB存在的情况下，即使有活性的NADPH生成同时存在，过氧化氢酶仍会失活，而谷胱甘肽浓度不受影响。本研究中获得的数据表明，使用不同的工具（MB），过氧化氢酶是过氧化氢解毒的活性酶，其完整性在很大程度上依赖于NADPH的充足生成。
