Floridi A, Morelli A, Floridi M
S TA NU. 1975 May-Jun;5(3):167-72.
This paper deals with the determination of red blood cell phosphorilated compounds, by automated ion exchange chromatography, in clinically healthy subjects, in two patients with hypochomic iron deficiency anemia and in four patients with macrocytic folic acid deficiency anemia. In the cases of nutritional anemia examined significant variations in the level of glycolitic phosphorilated compounds have been observed. The total acid-soluble phosphate content presents a marked increase in patients with anemia, compared with clinically healthy subjects. Very high increase in the 2,3 DPG, F1, 6DP, R5P, G1, 6DP and IMP red blood cell content is observed. On the contrary, ATP red blood cell concentration presents a marked decrease in all the patients.
本文采用自动离子交换色谱法,对临床健康受试者、两名低色素性缺铁性贫血患者和四名巨幼细胞性叶酸缺乏性贫血患者的红细胞磷酸化化合物进行了测定。在所检测的营养性贫血病例中,已观察到糖酵解磷酸化化合物水平存在显著差异。与临床健康受试者相比,贫血患者的总酸溶性磷酸盐含量显著增加。观察到2,3-二磷酸甘油酸(2,3 DPG)、果糖-1,6-二磷酸(F1, 6DP)、核糖-5-磷酸(R5P)、葡萄糖-1,6-二磷酸(G1, 6DP)和次黄嘌呤核苷酸(IMP)的红细胞含量大幅增加。相反,所有患者的红细胞ATP浓度均显著降低。