Frengova Ginka I, Simova Emilina D, Beshkova Dora M, Simov Zhelyasko I
Laboratory of Applied Mircobiology, Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv.
Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):805-10. doi: 10.1515/znc-2002-9-1009.
A Lactobacillus delbrueckii subsp. bulgaricus HP1 strain with high exopolysaccharide activity was selected from among 40 strains of lactic acid bacteria, isolated from kefir grains. By associating the Lactobacillus delbrueckii subsp. bulgaricus HP1 strain with Streptococcus thermophilus T15, Lactococcus lactis subsp. lactis C15, Lactobacillus helveticus MP12, and Sacharomyces cerevisiae A13, a kefir starter was formed. The associated cultivation of the lactobacteria and yeast had a positive effect on the exopolysaccharide activity of Lactobacillus delbrueckii subsp. bulgaricus HP1. The maximum exopolysaccharide concentration of the starter culture exceeded the one by the Lactobacillus delbrueckii subsp. bulgaricus HP1 monoculture by approximately 1.7 times, and the time needed to reach the maximum concentration (824.3 mg exopolysacharides/l) was shortened by 6 h. The monomer composition of the exopolysaccharides from the kefir starter culture was represented by glucose and galactose in a 1.0:0.94 ratio, which proves that the polymer synthesized is kefiran.
从开菲尔粒中分离出的40株乳酸菌中筛选出一株具有高胞外多糖活性的德氏乳杆菌保加利亚亚种HP1菌株。通过将德氏乳杆菌保加利亚亚种HP1菌株与嗜热链球菌T15、乳酸乳球菌乳酸亚种C15、瑞士乳杆菌MP12和酿酒酵母A13联合，形成了一种开菲尔发酵剂。乳酸菌和酵母的联合培养对德氏乳杆菌保加利亚亚种HP1的胞外多糖活性有积极影响。发酵剂培养物的最大胞外多糖浓度比德氏乳杆菌保加利亚亚种HP1单培养物的最大胞外多糖浓度高出约1.7倍，达到最大浓度（824.3 mg胞外多糖/升）所需的时间缩短了6小时。开菲尔发酵剂培养物中胞外多糖的单体组成以葡萄糖和半乳糖的比例为1.0:0.94表示，这证明合成的聚合物是开菲尔多糖。
