Molaei M M, Oloumi M M, Maleki M, Abshenas J
Department of Clinical Studies, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran.
J Vet Med A Physiol Pathol Clin Med. 2002 Sep;49(7):379-84. doi: 10.1046/j.1439-0442.2002.00467.x.
Injuries to the teat in dairy cows can result in partial or complete obstruction of the teat lumen. Different treatment techniques have been used to restore normal function in injured teats, one of which is autogenous mucosal grafts. The purpose of this study was to evaluate the vestibular mucosa as a replacement for teat mucosa in severe teat injuries. Sixteen teats of four healthy, mature, non-gravid Jersey cows were randomly divided into two equal groups. Under high epidural analgesia and after surgical preparation a 1 x 1.5 cm piece of teat mucosa was removed. In group 1, the defect was replaced by a 2 x 2.5 cm vestibular mucosa, whereas in group 2, the defect was left open. In both groups, a sterile disposable teat cannula was inserted into the teat cistern following surgery. To evaluate luminal diameter, double contrast radiography with constant air pressure was performed every 25 days till day 125, after which the animals were slaughtered and teats removed for histopathological study (H&E staining). On the basis of radiographic examination, luminal narrowing in group 2 was significantly more severe than in group 1. Histopathologically, the entire mucosal grafts of group 1 were taken and a good adhesion could be seen between the graft and the host epithelium. In group 2, severe submucosal fibrosis and mucosal papilloid hyperplasia resulted in severe narrowing of the teat cistern. According to the results of this study, it can be concluded that using vestibular mucosal grafts with temporary insertion of teat cannula can be considered as a method of treating teat mucosal injuries.
奶牛乳头损伤可导致乳头管腔部分或完全阻塞。人们采用了不同的治疗技术来恢复受损乳头的正常功能，其中一种是自体黏膜移植。本研究的目的是评估前庭黏膜作为严重乳头损伤中乳头黏膜替代物的效果。将4头健康、成熟、未怀孕的泽西奶牛的16个乳头随机分为两组，每组数量相等。在高位硬膜外镇痛和手术准备后，切除1×1.5 cm的乳头黏膜片。在第1组中，缺损处用2×2.5 cm的前庭黏膜替代，而在第2组中，缺损处保持开放。两组在术后均将无菌一次性乳头插管插入乳头池。为评估管腔直径，每隔25天进行一次恒压双重对比造影，直至第125天，之后宰杀动物并取出乳头进行组织病理学研究（苏木精-伊红染色）。根据影像学检查，第2组的管腔狭窄明显比第1组严重。组织病理学检查显示，第1组的整个黏膜移植片存活，移植片与宿主上皮之间可见良好的粘连。在第2组中，严重的黏膜下纤维化和黏膜乳头状增生导致乳头池严重狭窄。根据本研究结果，可以得出结论，使用前庭黏膜移植并临时插入乳头插管可被视为一种治疗乳头黏膜损伤的方法。
