Yagi F, Hiroyama H, Kodama S
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto Kagoshima 890-0065, Japan.
Glycoconj J. 2001 Oct;18(10):745-9. doi: 10.1023/a:1021193432577.
The complete amino acid sequence of Agrocybe cylindracea lectin was determined from the peptides obtained by chemical cleavages and enzymatic hydrolyses. The sequence shows 19.1% and 36.8% identity with those of human galectin-1 and Coprinus lectin-1, a fungal galectin, respectively. Seven residues, which are commonly found in carbohydrate recognizing domain (CRD) of galectins, were conserved. However, several insertions in the sequence, compared with those of human galectin-1 and Coprinus lectin-1, suggest that beta-strands S2, F3, and S4 and the loop structures between beta-strands F2 & S3 and F5 & S2 are different from those of galectins reported so far.
通过化学裂解和酶解获得的肽段确定了柱状田头菇凝集素的完整氨基酸序列。该序列与人半乳糖凝集素-1和真菌半乳糖凝集素鸡腿菇凝集素-1的序列分别具有19.1%和36.8%的同一性。在半乳糖凝集素的碳水化合物识别结构域（CRD）中常见的七个残基是保守的。然而，与人类半乳糖凝集素-1和鸡腿菇凝集素-1相比，该序列中的几个插入表明β链S2、F3和S4以及β链F2与S3和F5与S2之间的环结构与迄今为止报道的半乳糖凝集素不同。
