Hamprecht B, Kemper W, Amano T
Brain Res. 1976 Jan 9;101(1):129-35. doi: 10.1016/0006-8993(76)90993-8.
A bromodeoxyuridine resistant mutant of rat gliom line C6 grown in the presence of N6,02'-dibutyryl adenosine-3':5'-cyclic monophosphate, although inexcitable by electrical current, responds to iontophoretic application of acetylcholine by slow hyperpolarization. The sensitivity to acetylcholine is strongly reduced by excessive amounts of acetylcholine. The hyperpolarization response is inhibited by atropine or alpha-bungarotoxin, but not by D-tubocurarine. In the absence of acetylcholine some cells display spontaneous hyperpolarizations at regular intervals. The membrane resting potential is decreased if the concentration of external K+ is raised.
在N6,02'-二丁酰腺苷-3':5'-环磷酸存在下生长的大鼠胶质瘤细胞系C6的溴脱氧尿苷抗性突变体，虽然不能被电流激发，但对乙酰胆碱的离子导入应用会产生缓慢的超极化反应。过量的乙酰胆碱会大大降低对乙酰胆碱的敏感性。超极化反应被阿托品或α-银环蛇毒素抑制，但不被筒箭毒碱抑制。在没有乙酰胆碱的情况下，一些细胞会定期出现自发超极化。如果外部K+浓度升高，膜静息电位会降低。
