人参皂苷Rb1对培养的雪旺细胞增殖的影响。
Effects of Ginsenoside Rb1 on proliferation of Schwann cells in culture.
作者信息
Hu Xitang, Chen Xiaoxiang, Xiong Liangjian
机构信息
Department of Orthopaedics and Traumatology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
出版信息
Chin J Traumatol. 2002 Dec;5(6):365-8.
OBJECTIVE
To investigate the effects of Ginsenoside Rb(1) on the proliferation of Schwann cells in culture.
METHODS
Applying MTT assay and Thymidine incorporation assay, the effects of Ginsenoside Rb(1) on the proliferation of Schwann cells isolated from the sciatic nerve of adult rat were studied.
RESULTS
Ginsenoside Rb(1) (10 microg/ml) significantly induced Schwann cell proliferation, the effect was similar to NGF (50 microg/ml). At high concentrations of Ginsenoside Rb(1) (1 mg/ml), the proliferation of Schwann cells was significantly inhibited.
CONCLUSIONS
Ginsenoside Rb(1) at the optimal concentrations is found to be effective in inducing the proliferation of Schwann cells, but at higher concentrations the drug is cytotoxic for Schwann cells.
目的
研究人参皂苷Rb(1)对培养的雪旺细胞增殖的影响。
方法
应用MTT法和胸腺嘧啶核苷掺入法，研究人参皂苷Rb(1)对从成年大鼠坐骨神经分离的雪旺细胞增殖的影响。
结果
人参皂苷Rb(1)（10微克/毫升）显著诱导雪旺细胞增殖，其作用与神经生长因子（50微克/毫升）相似。在高浓度人参皂苷Rb(1)（1毫克/毫升）时，雪旺细胞的增殖受到显著抑制。
结论
发现最佳浓度的人参皂苷Rb(1)可有效诱导雪旺细胞增殖，但在较高浓度时该药物对雪旺细胞具有细胞毒性。
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