Sáez Carmen, Pereda Teresa, Borrero Juan J, Espina Agueda, Romero Francisco, Tortolero María, Pintor-Toro José A, Segura Dolores I, Japón Miguel A
Department of Pathology, Hospital Universitario Virgen del Rocío, Seville, 41013 Spain.
Oncogene. 2002 Nov 21;21(53):8173-7. doi: 10.1038/sj.onc.1205954.
Pituitary tumor-transforming gene (pttg) is a distinct proto-oncogene which is expressed in certain normal tissues with high proliferation rate and in a variety of tumors. PTTG is the vertebrate analog of yeast securins Pds1 and Cut2 with a key role in the regulation of sister chromatid separation during mitosis. Impairment of PTTG regulated functions is expected to lead to chromosomal instability and aneuploidy. Human pttg (hpttg) is abundantly expressed in Jurkat T lymphoblastic lymphoma cells but not in normal peripheral blood leukocytes. To obtain additional data on the potential role of hpttg in lymphomagenesis we selected 150 cases of lymphoid tumors for the assessment of hpttg expression in tumor tissues. Immunohistochemical studies on formalin-fixed, paraffin-embedded tissues revealed hPTTG in 38.8% of B-cell lymphomas, 70.2% of T-cell lymphomas, and 73.1% of Hodgkin's lymphomas. Among B-cell lymphomas, the most frequently immunostained tumors were plasma cell tumors, diffuse large cell lymphomas, and follicle center cell lymphomas. In Hodgkin's disease, immunoreactivity was mainly noted in Reed-Sternberg cells. In conclusion, the frequent overexpression of hpttg in many histological subtypes of lymphoma suggests the involvement of this proto-oncogene in lymphomagenesis.
垂体肿瘤转化基因（pttg）是一种独特的原癌基因，在某些增殖率高的正常组织以及多种肿瘤中均有表达。PTTG是酵母分离酶Pds1和Cut2在脊椎动物中的类似物，在有丝分裂期间姐妹染色单体分离的调控中起关键作用。预计PTTG调控功能受损会导致染色体不稳定和非整倍体。人pttg（hpttg）在Jurkat T淋巴细胞淋巴瘤细胞中大量表达，但在正常外周血白细胞中不表达。为了获得关于hpttg在淋巴瘤发生中潜在作用的更多数据，我们选择了150例淋巴肿瘤病例来评估肿瘤组织中hpttg的表达。对福尔马林固定、石蜡包埋组织的免疫组织化学研究显示，hPTTG在38.8%的B细胞淋巴瘤、70.2%的T细胞淋巴瘤和73.1%的霍奇金淋巴瘤中表达。在B细胞淋巴瘤中，免疫染色最频繁的肿瘤是浆细胞瘤、弥漫性大细胞淋巴瘤和滤泡中心细胞淋巴瘤。在霍奇金病中，免疫反应主要见于里德-斯腾伯格细胞。总之，hpttg在淋巴瘤的许多组织学亚型中频繁过度表达，提示该原癌基因参与了淋巴瘤的发生。
