Sáez C, Japón M A, Ramos-Morales F, Romero F, Segura D I, Tortolero M, Pintor-Toro J A
Departamento de Anatomía Patológica, Hospital Universitario Virgen del Rocío, 41013-Sevilla, Spain.
Oncogene. 1999 Sep 23;18(39):5473-6. doi: 10.1038/sj.onc.1202914.
The role of oncogenes in pituitary tumorigenesis remains elusive since few genetic changes have been identified so far in pituitary tumors. Pituitary tumor-transforming gene (pttg) has been recently cloned from rat GH4 pituitary tumor cells. We have previously isolated and characterized hpttg from human thymus. In the present study, we analyse the expression of hpttg mRNA in a series of human pituitary adenomas. We show that hpttg is highly expressed in the majority of pituitary adenomas while only very low levels of mRNA can be detected in normal pituitary gland by Northern blot analysis. hPTTG protein was immunolocalized mainly in the cytoplasm of adenoma cells. Other common extra-cranial malignant tumors were also analysed by immunohistochemistry. Interestingly, strong hPTTG immunoreactivity was detected in most adenocarcinomas of mammary and pulmonary origins.
由于目前在垂体肿瘤中仅发现了少数基因变化，因此癌基因在垂体肿瘤发生中的作用仍不清楚。垂体肿瘤转化基因（pttg）最近已从大鼠GH4垂体肿瘤细胞中克隆出来。我们之前已从人胸腺中分离并鉴定了hpttg。在本研究中，我们分析了一系列人垂体腺瘤中hpttg mRNA的表达。我们发现，hpttg在大多数垂体腺瘤中高度表达，而通过Northern印迹分析在正常垂体中仅能检测到极低水平的mRNA。hPTTG蛋白主要免疫定位于腺瘤细胞的细胞质中。我们还通过免疫组织化学分析了其他常见的颅外恶性肿瘤。有趣的是，在大多数乳腺和肺源性腺癌中检测到强烈的hPTTG免疫反应性。
