Cancino-Díaz Juan C, Reyes-Maldonado Elba, Bañuelos-Pánuco Carmen A, Jiménez-Zamudio Luis, García-Latorre Ethel, León-Dorantes Gladys, Blancas-González Fernando, Paredes-Cabrera Giovanna, Cancino-Díaz Mario E
Departamento de Inmunología Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, México D.F. 11340, México.
J Invest Dermatol. 2002 Nov;119(5):1114-20. doi: 10.1046/j.1523-1747.2002.19509.x.
Although several cytokines and their receptors have been involved in the development of psoriasis, the etiology is still unknown. In this study we looked for genes possibly involved in the disease by the reverse transcription-polymerase chain reaction differential display technique in lesional and nonlesional skin biopsies from psoriatic patients. We found the mRNA of the alpha1 chain of the interleukin-13 receptor expressed differentially in psoriatic biopsies. By reverse transcription-polymerase chain reaction, we confirmed an overexpression of the alpha1 chain of the IL-13 receptor and alpha chain of the interleukin-4 receptor mRNA in lesional skin psoriatic biopsies, when compared with skin biopsies from healthy subjects (p<0.01). The nonlesional skin obtained from a region close to a lesional zone in psoriatic patients presented also an overexpression of these mRNA in 50% of the samples. Interleukin-13 and interleukin-4 were not detected either as mRNA or as the proteins in any of the biopsies from psoriatic patients or healthy subjects. A monoclonal antibody to the alpha1 chain of the interleukin-13 receptor detected the receptor in the epidermal keratinocytes of psoriatic patients and of healthy subjects; however, the positive antibody reaction was stronger in skin tissue from healthy subjects than in psoriatic lesional skin tissue (p<0.01), although the mRNA was overexpressed. As interleukin-13 is a pleiotropic immunoregulatory cytokine with a variety of effects on different cell types, including monocytes, B lymphocytes, mast cells, and keratinocytes, we suggest, based on our results, that the interleukin-13 receptor possibly plays an important part in the early inflammatory process of psoriasis; however, its function is lost in the psoriatic keratinocytes.
尽管多种细胞因子及其受体与银屑病的发病机制有关，但其病因仍不清楚。在本研究中，我们采用逆转录-聚合酶链反应差异显示技术，在银屑病患者的皮损和非皮损皮肤活检组织中寻找可能与该病相关的基因。我们发现白细胞介素-13受体α1链的mRNA在银屑病活检组织中表达存在差异。通过逆转录-聚合酶链反应，我们证实与健康受试者的皮肤活检组织相比，银屑病皮损活检组织中白细胞介素-13受体α1链和白细胞介素-4受体α链的mRNA表达上调（p<0.01）。从银屑病患者皮损区附近获取的非皮损皮肤样本中，50%也存在这些mRNA的过表达。在银屑病患者或健康受试者的任何活检组织中，均未检测到白细胞介素-13和白细胞介素-4的mRNA或蛋白质。一种针对白细胞介素-13受体α1链的单克隆抗体在银屑病患者和健康受试者的表皮角质形成细胞中均检测到该受体；然而，尽管mRNA过表达，但健康受试者皮肤组织中的阳性抗体反应比银屑病皮损皮肤组织更强（p<0.01）。由于白细胞介素-13是一种多效性免疫调节细胞因子，对包括单核细胞、B淋巴细胞、肥大细胞和角质形成细胞在内的不同细胞类型具有多种作用，基于我们的研究结果，我们认为白细胞介素-13受体可能在银屑病的早期炎症过程中起重要作用；然而，其功能在银屑病角质形成细胞中丧失。
