Dias Nathalie, Stein C A
Columbia University, New York, NY 10032, USA.
Eur J Pharm Biopharm. 2002 Nov;54(3):263-9. doi: 10.1016/s0939-6411(02)00060-7.
Antisense oligonucleotides have been widely used to specifically and selectively downregulate gene expression at the messenger RNA level. Even though oligonucleotides are commonly used in laboratories and clinical trials, they can induce non-specific effects that can lead to misinterpretation of experimentally-derived results. This review summarizes precautions one should take when using oligonucleotides. In addition, the role of one oligonucleotide, G3139, which is targeted to the coding region of bcl-2 messenger RNA, in inhibiting tumor progression in vitro and in clinical trials, is described.
反义寡核苷酸已被广泛用于在信使核糖核酸水平特异性和选择性地下调基因表达。尽管寡核苷酸在实验室和临床试验中普遍使用，但它们可诱导非特异性效应，从而导致对实验结果的错误解读。本综述总结了使用寡核苷酸时应采取的预防措施。此外，还描述了一种靶向bcl-2信使核糖核酸编码区的寡核苷酸G3139在体外和临床试验中抑制肿瘤进展的作用。
